The
Project
The major challenge for PureCell is to bridge the ‘generation gap’ between the more conventional, «low-throughput» and the recent high-throughput technological/methodological approaches in cellular analytics.
The installation of state-of-the-art cell sorting and flow cytometry equipment combined with a live-cell metabolic kinetic infrastructure offer PureCell partnership researchers the possibility to isolate homogeneous populations of cells and extracellular particles for use in a variety of downstream assays and projects aimed at unraveling disease pathogenesis and identifying novel potential therapeutic targets. Additionally, it facilitates the study of cellular bioenergetics in a non-invasive fashion, which will contribute to understanding the metabolic state of pathologic cell populations, bystander cells and their interactions.In the context of PureCell, novel methodologies/assays will be delineated and relayed to our comprehensive characterization of biological phenomena/processes in Life Sciences. Importantly, the proposed solutions and/or pipelines should establish frameworks and constitute a reference for the entire ecosystem of interested stakeholders (academia, pharma and biotechnology industry, public and private health system). In the long-term, PureCell will strengthen the foundations of the Greek Research Area by providing a critical mass of expertise in advanced cellular analytics. Critically, the Cell Analysis Facility is openly accessible for services, research and educational applications in the field, in a form allowing extensibility and further advancements.
Instrumentation
The Cell Analysis Facility is equipped with one high-speed cell sorter (BD FACSAria III), one full-spectrum cytometer (Cytek Northern Lights) and one real-time cell metabolic analyzer (Agilent Seahorse XFe96).
Fees
The use of Cell Analysis Facility is free of charge. Our partners have the responsibility to carry their own reagents for the proposed assays.
Eligibility for access to Cell Analysis Facility
PureCell aims towards the establishment of a National Open Infrastructure for Advanced Cellular Analytics in biomedical research that will be relevant to not only INAB|CERTH scientists and collaborators but the academic sector at large, that will thus have increased opportunities for cutting-edge research. The Cell Analysis Facility is open to anyone, who is interested in using our infrastructure, upon request.
Funding & Acknowledgements
Please acknowledge use of the facility in all publications.
Suggested text: Flow cytometry was performed at the Institute of Applied Biosciences (INAB)|Centre for Research and Technology Hellas (CERTH), supported by the Hellenic Foundation for Research and Innovation (H.F.R.I.), grant reference: HFRI-FM17C3-2810 and project: PureCell-Towards Precision Medicine: Advanced cellular analytics in biomedical research.
